Sample Preparation and Shipment
A shipping address will be provided once submitted plate(s) are approved by a reviewer. Plate approval is required before shipping samples. Samples that are shipped without approval will incur storage and/or return shipping and labor fees.
Sample Preparation:
DNAs should be dissolved in TE (10 mM Tris, EDTA 1.0 or 0.1 mM, pH 7.5-8). The elution buffer included in Qiagen DNA extraction kits (AE, which is 0.1XTE) is also fine. DNA concentrations do not need to be standardized across the plate. To avoid additional processing fees, samples should be within the range of concentrations stated in the User guide section: Submitting plated DNAs for genotyping.
The “blank” well(s) of plates should be empty (devoid of liquid). These wells are used as negative controls for DNA sequencing. On arrival, plates are checked to confirm that this well is empty. If liquid is present in the “blank”, plates may be rejected.
Plates and Sealing Methods:
Samples should be shipped in semi-skirted, full or non-skirted 96-well PCR plates. We recommend Eppendorf twin.tec, semi-skirted plates, #951020303 with caps from ABGene #AB-0783. Samples sent in non-standard plasticware (tubes or deep-well plates) will be rejected and/or incur additional fees. Plates should be sealed well with caps or heat seals to avoid leaking and sample cross-contamination. All other methods of sealing (including silicone mats and adhesive seals) will result in rejection of samples.
Shipping Methods:
We recommend shipping samples at ambient temperature. Shipping on ice packs or dry ice is acceptable but do not ship samples on wet ice. Wrap plates well in bubble wrap or other cushioning to prevent plate damage during transit. Plates should be labeled clearly with your plate ID number, Project, and Plate name and date. This information should also be printed and included as an enclosure inside the box.
Version 2 Updated 2016-02-12